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Bacteriostatic water (BAC water): Preferred for multi-dose vials. The 0.9% benzyl alcohol preservative inhibits microbial growth, extending shelf life to 4–6 weeks refrigerated. Sterile water: Suitable for single-use reconstitution. Use within 72 hours. Dilute acetic acid (0.1%): For hydrophobic or aggregation-prone peptides DMSO: For poorly soluble peptides — use minimal volume and dilute further with aqueous buffer Centrifuge the sealed vial at low speed (500–1000 rpm) for 30 seconds to pellet any powder from the walls or cap Wipe the septum with a 70% isopropyl alcohol swab and allow to dry Draw up the desired volume of solvent using a sterile syringe Inject slowly into the vial, aiming the stream at the glass wall — not directly onto the powder Gently swirl the vial. Do not vortex or shake vigorously. Allow to sit for 5–10 minutes at room temperature if not fully dissolved Do not use if the solution remains cloudy or contains visible particles Label immediately with: peptide name, concentration, date, initials Store at 2–8°C refrigerator Do not freeze reconstituted solutions Discard at first sign of cloudiness, color change, or at expiry
Lab Protocols 5 min read2024-09-15
How to Reconstitute Lyophilized Peptides
Proper reconstitution is critical for research accuracy. This step-by-step guide covers solvent selection, concentration calculations, and storage after reconstitution.
Why Reconstitution Technique Matters
Lyophilized peptides must be reconstituted before use. The process seems simple — add solvent, mix — but errors at this step can result in inaccurate concentrations, aggregated peptide, or degraded material.
Choosing the Right Solvent
The choice of reconstitution solvent depends on the peptide's properties:
Always consult the peptide's technical datasheet when available.
Step-by-Step Reconstitution Protocol
Calculating Concentration
Use our Dosing Calculator to determine exact volumes and concentrations.
Example: 5 mg peptide + 2 mL BAC water = 2,500 mcg/mL solution